Hanash, Samir

Open Protocols

Protocol Name Investigatory Role Biomarkers Data Collections
A Biomarker Bakeoff in Early Stage Pancreatic Cancer Involved 11 0
Affinity-based strategies to fast track development of colon cancer biomarkers Involved 0 0
Biomarkers of Risk for Colorectal Neoplasia (Team Project #2) Involved 0 0
Breast Reference Set-Hanash (2024) Leading 0 0
Indeterminate pulmonary nodule biomarker clinical utility trial Involved 0 0
LTP2 Ref Set App: Wilson MDACC Images (2023) Involved 0 0
Pancreatic Cancer Bake Off 2 Involved 4 0
Phase 2 and 3 Validation of Protein Biomarkers for the Early Detection of Breast Cancer Discovered Using a Mass Spectrometry-Based Platform Coordinating 0 0
Pre-diagnostic Pancreatic Cancer Set Aside Project Involved 25 0
The Development of Targeted Therapeutic Approaches for Pancreatic Cancer Involved 0 0

Closed Protocols

Protocol Name Investigatory Role Biomarkers Data Collections
EDRN-WHI Pre-Clinical Colon Ca Specimens Leading 0 1
Identification of biomarkers for lung cancer in never smokers Involved 4 1
Pancreatic Reference Set Application: Samir Hanash-MD Anderson (2017) Leading 8 0
Preliminary Validation of Biomarkers for the Detection of Colorectal Adenomas (Team Project #1) Involved 1 0
Standard Specimen Reference Set: Lung Involved 0 0
Validation of Protein Markers for Lung Cancer Using CARET Sera and Proteomics Techniques Leading 16 1


Publication NamePubMed IDJournal
2-D or not 2-D--is there a future for 2-D gels in proteomics? Insights from the York proteomics meeting. 11678032 Proteomics
A database of protein expression in lung cancer. 11721642 Proteomics
A distinct repertoire of autoantibodies in hepatocellular carcinoma identified by proteomic analysis. 12096119 Mol Cell Proteomics
A functional annotation of subproteomes in human plasma. 16104058 Proteomics
A meeting of minds in proteomics. 11822353 Proteomics
A minimal critical region of the 8p22-23 amplicon in esophageal adenocarcinomas defined using sequence tagged site-amplification mapping and quantitative polymerase chain reaction includes the GATA-4 gene. 10728696 Cancer Res
A mouse plasma peptide atlas as a resource for disease proteomics. 18522751 Genome Biol
A mouse to human search for plasma proteome changes associated with pancreatic tumor development. 18547137 PLoS Med
A proteomic approach to the identification of lung cancer markers. 11790896 Dis Markers
A proteomics platform combining depletion, multi-lectin affinity chromatography (M-LAC), and isoelectric focusing to study the breast cancer proteome. 21513341 Anal Chem
A wide range of protein isoforms in serum and plasma uncovered by a quantitative intact protein analysis system. 16047307 Proteomics
Accurate molecular classification of human cancers based on gene expression using a simple classifier with a pathological tree-based framework. 14578198 Am J Pathol
An immune response manifested by the common occurrence of annexins I and II autoantibodies and high circulating levels of IL-6 in lung cancer. 11504947 Proc Natl Acad Sci U S A
Application of proteomics to cancer early detection. 22157286 Cancer J
Biomedical applications of two-dimensional electrophoresis using immobilized pH gradients: current status. 10786892 Electrophoresis
Challenges in deriving high-confidence protein identifications from data gathered by a HUPO plasma proteome collaborative study. 16525410 Nat Biotechnol
Cleavage of endoplasmic reticulum proteins in hepatocellular carcinoma: Detection of generated fragments in patient sera. 16762624 Gastroenterology
Comprehensive proteomic profiling of aldehyde dehydrogenases in lung adenocarcinoma cell lines. 22110949 Int J Proteomics
Computational Proteomics Analysis System (CPAS): an extensible, open-source analytic system for evaluating and publishing proteomic data and high throughput biological experiments. 16396501 J Proteome Res
Concordant release of glycolysis proteins into the plasma preceding a diagnosis of ER+ breast cancer. 22367215 Cancer Res
Confounding effects of hormone replacement therapy in protein biomarker studies. 21037107 Cancer Epidemiol Biomarkers Prev
Contribution of protein fractionation to depth of analysis of the serum and plasma proteomes. 17696519 J Proteome Res
Contributions of proteome profiling to the molecular analysis of cancer. 12625782 Technol Cancer Res Treat
Discordant protein and mRNA expression in lung adenocarcinomas. 12096112 Mol Cell Proteomics
Discovery of cancer biomarkers through the use of mouse models. 17320282 Cancer Lett
Disease proteomics. 12634796 Nature
Distinctive serum protein profiles involving abundant proteins in lung cancer patients based upon antibody microarray analysis. 16117833 BMC Cancer
Emerging molecular biomarkers--blood-based strategies to detect and monitor cancer. 21364687 Nat Rev Clin Oncol
Evaluation of known oncoantibodies, HER2, p53, and cyclin B1, in prediagnostic breast cancer sera. 22715141 Cancer Prev Res (Phila)
Expression of matrix metalloproteinase 9 (MMP-9/gelatinase B) in adenocarcinomas strongly correlated with expression of immune response genes. 12954092 In Silico Biol
Gene expression patterns define pathways correlated with loss of differentiation in lung adenocarcinomas. 12681502 FEBS Lett
Gene-expression profiles predict survival of patients with lung adenocarcinoma. 12118244 Nat Med
Global and specific translational control by rapamycin in T cells uncovered by microarrays and proteomics. 11943782 J Biol Chem
Global profiling of gene expression in cancer using genomics and proteomics. 11804268 Curr Opin Mol Ther
Global profiling of the cell surface proteome of cancer cells uncovers an abundance of proteins with chaperone function. 12493773 J Biol Chem
Harnessing immunity for cancer marker discovery. 12511908 Nat Biotechnol
Harnessing the immune response for cancer detection. 21454417 Cancer Epidemiol Biomarkers Prev
High-throughput genomic technology in research and clinical management of breast cancer. Plasma-based proteomics in early detection and therapy. 17184556 Breast Cancer Res
Identification of 14-3-3 theta as an antigen that induces a humoral response in lung cancer. 18089831 Cancer Res
Identification of a Specific Vimentin Isoform That Induces an Antibody Response in Pancreatic Cancer. 18769604 Biomark Insights
Identification of androgen-regulated genes in the prostate cancer cell line LNCaP by serial analysis of gene expression and proteomic analysis. 11721644 Proteomics
Identification of beta-tubulin isoforms as tumor antigens in neuroblastoma. 11051243 Clin Cancer Res
Identification of tumor-associated antigens using proteomics. 12625784 Technol Cancer Res Treat
Increased C-CRK proto-oncogene expression is associated with an aggressive phenotype in lung adenocarcinomas. 12970743 Oncogene
Increased throughput and reduced carryover of mass spectrometry-based proteomics using a high-efficiency nonsplit nanoflow parallel dual-column capillary HPLC system. 18512973 J Proteome Res
Innovative proteomic approaches for cancer biomarker discovery. 17907570 Biotechniques
Intact-protein analysis system for discovery of serum-based disease biomarkers. 21468941 Methods Mol Biol
Integral protein microarrays for the identification of lung cancer antigens in sera that induce a humoral immune response. 17916591 Mol Cell Proteomics
Integrated mass spectrometry-based analysis of plasma glycoproteins and their glycan modifications. 21372808 Nat Protoc
Integrating cancer genomics and proteomics in the post-genome era. 11788993 Proteomics
Integration of proteomics into systems biology of cancer. 22407608 Wiley Interdiscip Rev Syst Biol Med
Isoelectric focusing nonporous RP HPLC: a two-dimensional liquid-phase separation method for mapping of cellular proteins with identification using MALDI-TOF mass spectrometry. 10740846 Anal Chem
Isoform analysis of LC-MS/MS data from multidimensional fractionation of the serum proteome. 18419151 J Proteome Res
Lung cancer signatures in plasma based on proteome profiling of mouse tumor models. 21907921 Cancer Cell
Making sense of microarray data to classify cancer. 14597962 Pharmacogenomics J
Microarrays of tumor cell derived proteins uncover a distinct pattern of prostate cancer serum immunoreactivity. 14595819 Proteomics
Microparticles from ovarian carcinomas are shed into ascites and promote cell migration. 22315094 Int J Gynecol Cancer
Mining gene expression databases for association rules. 12499296 Bioinformatics
Mining the plasma proteome for cancer biomarkers. 18385731 Nature
Mining the plasma proteome for disease applications across seven logs of protein abundance. 21062094 J Proteome Res
Molecular analysis of cancer using DNA and protein microarrays. 12908549 Adv Exp Med Biol
Molecular profiling of pancreatic adenocarcinoma and chronic pancreatitis identifies multiple genes differentially regulated in pancreatic cancer. 12750293 Cancer Res
Molecular profiling of the immune response in colon cancer using protein microarrays: occurrence of autoantibodies to ubiquitin C-terminal hydrolase L3. 14595809 Proteomics
Mouse model of human ovarian endometrioid adenocarcinoma based on somatic defects in the Wnt/beta-catenin and PI3K/Pten signaling pathways. 17418409 Cancer Cell
Multi-dimensional liquid phase based separations in proteomics. 12659729 J Chromatogr B Analyt Technol Biomed Life Sci
Occurrence of autoantibodies to annexin I, 14-3-3 theta and LAMR1 in prediagnostic lung cancer sera. 18794547 J Clin Oncol
Operomics: integrated genomic and proteomic profiling of cells and tissues. 15251063 Brief Funct Genomic Proteomic
Operomics: molecular analysis of tissues from DNA to RNA to protein. 11097332 Clin Chem Lab Med
Overexpression of oncoprotein 18 correlates with poor differentiation in lung adenocarcinomas. 12644570 Mol Cell Proteomics
Overview of the HUPO Plasma Proteome Project: results from the pilot phase with 35 collaborating laboratories and multiple analytical groups, generating a core dataset of 3020 proteins and a publicly-available database. 16104056 Proteomics
Plasma proteome profiles associated with inflammation, angiogenesis, and cancer. 21589862 PLoS One
Plasma proteome profiling of a mouse model of breast cancer identifies a set of up-regulated proteins in common with human breast cancer cells. 18311905 J Proteome Res
Profiling of pathway-specific changes in gene expression following growth of human cancer cell lines transplanted into mice. 12844362 Genome Biol
Profiling of the cell surface proteome. 14625857 Proteomics
Protein based microarrays: a tool for probing the proteome of cancer cells and tissues. 11721639 Proteomics
Protein profiles associated with survival in lung adenocarcinoma. 14573703 Proc Natl Acad Sci U S A
Proteomic analysis of cytokeratin isoforms uncovers association with survival in lung adenocarcinoma. 12192603 Neoplasia
Proteomic analysis of eIF-5A in lung adenocarcinomas. 12687616 Proteomics
Proteomic analysis of lung adenocarcinoma: identification of a highly expressed set of proteins in tumors. 12114434 Clin Cancer Res
Proteomic approaches for cancer biomarker discovery in plasma. 17941811 Expert Rev Proteomics
Proteomic approaches within the NCI early detection research network for the discovery and identification of cancer biomarkers. 11708463 Ann N Y Acad Sci
Proteomics approaches to uncover the repertoire of circulating biomarkers for breast cancer. 12882525 J Mammary Gland Biol Neoplasia
Proteomics in early detection of cancer. 11568117 Clin Chem
Proteomics-based identification of protein gene product 9.5 as a tumor antigen that induces a humoral immune response in lung cancer. 11691811 Cancer Res
Proteomics-based identification of RS/DJ-1 as a novel circulating tumor antigen in breast cancer. 11705844 Clin Cancer Res
Quantitative analysis of acrylamide labeled serum proteins by LC-MS/MS. 16889424 J Proteome Res
RANTES expression is a predictor of survival in stage I lung adenocarcinoma. 12473593 Clin Cancer Res
Targets of the tumor suppressor miR-200 in regulation of the epithelial-mesenchymal transition in cancer. 21987723 Cancer Res
The gene for the axonal cell adhesion molecule TAX-1 is amplified and aberrantly expressed in malignant gliomas. 11280781 Cancer Res
The hepatocyte nuclear factor 3 alpha gene, HNF3alpha (FOXA1), on chromosome band 14q13 is amplified and overexpressed in esophageal and lung adenocarcinomas. 12234996 Cancer Res
The Human Proteome Organization: a mission to advance proteome knowledge. 12169681 Mol Cell Proteomics
The major CD9 and CD81 molecular partner. Identification and characterization of the complexes. 11278880 J Biol Chem
Transforming properties of a Q18-->E mutation of the microtubule regulator Op18. 12242154 Cancer Cell
Tumor microenvironment-derived proteins dominate the plasma proteome response during breast cancer induction and progression. 21653680 Cancer Res
Two messenger RNAs and five isoforms for Po66-CBP, a galectin-8 homolog in a human lung carcinoma cell line. 11675018 Gene
Ubiquitin COOH-terminal hydrolase 1: a biomarker of renal cell carcinoma associated with enhanced tumor cell proliferation and migration. 17200335 Clin Cancer Res
Unraveling the complex proteome for biomarker discovery in gastrointestinal and liver diseases. 17064703 Gastroenterology
Virtual genome scan: a tool for restriction landmark-based scanning of the human genome. 11483587 Genome Res


Proteomics for cancer detection and therapy
We focus on the development and application of integrated approaches to the molecular profiling of cancer, with particular emphasis on proteomics. Our individual projects focus on molecular profiling of lung, colon, breast, ovarian and other cancers, with potential applications in early diagnosis or therapy. We have a particular interest in the discovery and validation of protein markers in serum and/or tissue for early diagnosis of cancer. Dr. Hanash relocated from the University of Michigan Medical School to the Fred Hutchinson Cancer Research Center in August 2004 to lead a new program in Molecular Diagnostics.

To update protocols, publications, biomarkers, or science data, please contact the Data Management and Coordinating Center.

Photograph of Hanash, Samir
The University of Texas MD Anderson Cancer Center
M.D., Ph.D.
Person ID
EDRN Title
EDRN Principal Investigator
To update contact information, please visit the Data Management and Coordinating Center .