Breast Reference Set Application: Chris Li-FHCRC (2013)

Abbreviated Name

Breast Ref Set App: Li (2013)

Lead Investigator

Li, Christopher — Fred Hutchinson Cancer Center

Coordinating Investigator

Feng, Ziding — Fred Hutchinson Cancer Center

Involved Investigators

Abstact

Aims

Targeted LC-MS/MS: Electro-spray ionization (ESI) will be performed and MS acquisition will be performed in multiple reaction monitoring (MRM) mode following identical procedures used in past studies (21). An Agilent 6410 triple quad MS system equipped with an Agilent 1200 UPLC system will be utilized. Each MS acquisition for the serum samples (0.050 mL each) will target the 11-marker panel of metabolites, each of which has a 13C or D-labeled internal standard to provide for reliable quantitation of the absolute metabolite concentrations in blood. Appropriate separation conditions for positive and negative mode detection will be performed (21, 33). Data Analysis Plan: Welch’s t-tests will be performed to determine if observed differences in etabolite signal intensities between invasive cancer cases and benign disease without atypia controls reach statistical significance (p<0.05, corrected using the false discovery rate method). ROC curves will be generated for individual candidate markers to assess sensitivity and specificity at all possible marker cut-point levels.

Analytic Method

Biomarkers

Data Collections

No data available at this time for this protocol

Team Project

Protocol ID

383

Protocol Type

Reference Set

Field of Research

Metabolomics

Collaborative Group

Breast and Gynecologic Cancers Research Group

Cancer Types

Malignant neoplasm of breast