3D pathology-guided microdissection.
Abstract
Traditional micro- and macro-dissection techniques enable the extraction of localized regions in thin tissue sections for molecular analysis. Despite the growing use of 3D microscopy, analogous methods for volumetric microdissection are lacking. We have developed a 3D microdissection method based on computer numerical controlled (CNC) milling integrated with open-top light-sheet microscopy. We demonstrate the ability to study tumor evolution along convoluted 3D branching architectures, which is inaccessible to 2D methods.
EDRN PI Authors
- (None specified)
Medline Author List
- Baraznenok E
- Bishop KW
- Brenes D
- Figiel S
- Gao G
- Grady WM
- Haffner MC
- Hamdy FC
- Han Q
- Hsieh HC
- Konnick EQ
- Liu JTC
- Mills IG
- Paulson TG
- Pritchard CC
- Rao SR
- Reddi DM
- Reder NP
- Serafin R
- True LD
- Valk JE
- Wang R
- Woodcock DJ
- Yan R