DNA copy number gains in malignant pleural mesothelioma.
Abstact
Malignant pleural mesothelioma (MPM) is a highly aggressive tumor with an extremely poor prognosis. The incidence of MPM is increasing as a result of widespread exposure to asbestos. The molecular pathogenesis of MPM remains unclear. The present study analyzed the frequency of various genomic copy number gains (CNGs) in MPM using reverse transcription-quantitative polymerase chain reaction. A total of 83 primary MPMs and 53 primary lung adenocarcinomas were analyzed to compare the CNGs of <i>EGFR, KRAS, MET, FGFR1</i> and <i>SOX2</i>. In MPM, the CNGs of <i>EGFR, KRAS, MET, FGFR1</i> and <i>SOX2</i> were detected in 12 (14.5%), 8 (9.6%), 5 (6.0%), 4 (4.8%) and 1 (1.2%) of the samples, respectively. In lung adenocarcinomas, the CNGs of <i>EGFR, KRAS, MET, FGFR1</i> and <i>SOX2</i> were detected in 21 (39.6%), 12 (22.6%), 5 (9.4%), 10 (18.9%) and 0 (0.0%) of the samples, respectively. The CNGs of <i>EGFR, KRAS</i> and <i>FGFR1</i> were significantly less frequent in the MPMs compared with the lung adenocarcinomas (P=0.0018, 0.048 and 0.018, respectively). Overall, the MPMs exhibited these CNGs less frequently compared with the lung adenocarcinomas (P=0.0002). The differences in CNGs between the two tumor types suggested that they are genetically different.
Authors
- Aoe K
- Asano H
- Fujimoto N
- Furukawa M
- Hashida S
- Hayashi T
- Kishimoto T
- Miyoshi S
- Okabe K
- Pass HI
- Shien K
- Soh J
- Toyooka S
- Tsukuda K
- Yamamoto H