Bioelectronic DNA detection of human papillomaviruses using eSensor: a model system for detection of multiple pathogens.

Abstract

We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor) in identifying multiple pathogens.

Two chips were spotted with capture probes consisting of DNA oligonucleotide sequences specific for HPV types. Electrically conductive signal probes were synthesized to be complementary to a distinct region of the amplified HPV target DNA. A portion of the HPV L1 region that was amplified by using consensus primers served as target DNA. The amplified target was mixed with a cocktail of signal probes and added to a cartridge containing a DNA chip to allow for hybridization with complementary capture probes.

Two bioelectric chips were designed and successfully detected 86% of the HPV types contained in clinical samples.

This model system demonstrates the potential of the eSensor platform for rapid and integrated detection of multiple pathogens.

Authors
  • Blackburn GF
  • Chan V
  • Chen YP
  • Farkas DH
  • Miller DL
  • Reeves WC
  • Unger ER
  • Vernon SD
PubMed ID
Appears In
BMC Infect Dis, 2003, 3