Proteomics Biomarker Development Laboratory

Abbreviated Name
Proteomics Biomarker Development Laboratory
Lead Investigator
No lead investigator
Coordinating Investigator
No coordinating investigator
Involved Investigators
  • No involved investigators

Abstact

The Biomarker Development Laboratory is focused on the application of a proteomic approach for the development of cancer biomarkers. The laboratory will apply technologies for protein analysis to the detection and identification of proteins secreted by tumor cells and protein antigens that induce a humoral response in tumor s. The initial targeted tumor types for the identification of potential biomarker proteins are: Colon, esophagus, ovary, lung, breast and liver. The proposed studies will make heavy utilization of two-dimensional (2-D) protein analysis, of protein identification technologies and of database capabilities that have been developed by the applicant group. The objectives of this research are to 1. Identify tumor antigens that induce a humoral response; 2. Identify tumor secreted proteins and develop a database; 3. Preliminarily validate the potential utility of markers for early cancer detection. Sixty ml of blood will be obtained from healthy normals, and from small cell lung cancer, hepatoma, esophageal, and breast cancer patients, and from ovarian, colon, and non-small cell lung cancer patients prior to surgical resection and six to ten weeks after resection. Demographic data, sera and DNA will be used in 2-D protein analysis to identify new or altered proteins. The initial validation will be the assay of the presence or absence of a given protein in a large sample cohort from the samples collected from these patients. There is no specified age range, although the diseases under study do not occur in children. No subjects will be excluded on the basis of race or gender. Women of child bearing age will be studied. Linkable demographic data will be held separately from the bar-coded serum or DNA samples. Serum and DNA sample will be identifiable only by subject identification number. Full written informed consent will be obtained from patients.

Aims

No aims available.

Analytic Method

No analytic method available.

Publications

  • No publications available at this time for this protocol.

Biomarkers

  • No biomarkers available at this time for this protocol.

Data Collections

  • No data collections available at this time for this protocol.
Protocol ID
53
Protocol Type
Single
Field of Research
UNKNOWN
Collaborative Group
(No collaborative group)
Cancer Types
  • (No cancer types specified)

Associated Forms