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You are here: Home / Resources / EDRN Standard Operating Procedures (SOP) / Assays / Immunohistochemistry / Immunoperoxidase Automated Procedure (Ventana)

Immunoperoxidase Automated Procedure (Ventana)

  1. Cut tissue sections at 3-5 microns and mount on appropriate control box slides.
  2. Bake slides for 30 minutes in 70°C - 90°C oven.
  3. Label slides with barcode labels indicating antibody.
  4. Place slides inside Ventana stainer and load all necessary antibodies and reagents.
  5. Start machine.
  6. Remove slides when finished and wash in warm soapy water (Dawn concentrate dishwashing detergent) to remove oil coating.
  7. Counter stain with Gills I hematoxylin for 1 minute then blue in Lithium Carbonate solution.
  8. Wash in tap water 2-5 minutes.
  9. Dehydrate slides.

    Rinse 15-20 dips in

    • 2 changes 95% ETOH
    • 2 changes ABS ETOH
    • 3 changes Xylene
  10. Coverslip on automatic coverslipper.
Announcement 04/26/2017

A new funding opportunity associated with the Beau Biden Cancer Moonshot Initiative, Integration and Validation of Emerging Technologies to Accelerate Cancer Research, has been released and is due May 10, 2017. Click here for more information.

Announcement 03/14/2017

Thank you to everyone who made the March 2017 EDRN Steering Committee Meeting a success. The next EDRN Steering Committee Meeting will be on September 12-14, 2017, in Seattle, WA.

EDRN Founder Honored

Dr. Sudhir Srivastava was honored with the Distinguished Service Award from the American Pancreatic Association at the group's annual meeting this year, for his outstanding commitment to pancreatology.