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Immunohistochemistry

Immunofluorescence Staining
Direct immunofluorescence method is used to detect the deposit of immunoglobulins, complement components, fibrinogen, etc. in tissues. This technique is usually performed on frozen sections. The primary antibody is conjugated to fluorescein binds directly with the antigen and can be detected by the fluorescent tag using a fluorescent microscope.
Immunoperoxidase Staining
Pre-staining informational manual and the protocol documents.
Immunoperoxidase Automated Procedure (Ventana)
Immunoperoxidase Protocol for Dako Autostainer
Acknowledgements
These SOPs were originally submitted by Sanford Stass, M.D. of the University of Maryland School of Medicine and William Grizzle of the University of Alabama at Birmingham School of Medicine. Modifications may have been made upon extensive discussions among the members of the EDRN Reference Laboratories and other scientific staff derived from the various groups within EDRN.
Announcement 06/30/2017

The next EDRN Steering Committee Meeting is from September 12-14, 2017, in Seattle WA. Please click here to register and find out more information about the meeting.

Announcement



EDRN Founder Honored

Dr. Sudhir Srivastava was honored with the Distinguished Service Award from the American Pancreatic Association at the group's annual meeting this year, for his outstanding commitment to pancreatology.