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Immunohistochemistry

Immunofluorescence Staining
Direct immunofluorescence method is used to detect the deposit of immunoglobulins, complement components, fibrinogen, etc. in tissues. This technique is usually performed on frozen sections. The primary antibody is conjugated to fluorescein binds directly with the antigen and can be detected by the fluorescent tag using a fluorescent microscope.
Immunoperoxidase Staining
Pre-staining informational manual and the protocol documents.
Immunoperoxidase Automated Procedure (Ventana)
Immunoperoxidase Protocol for Dako Autostainer
Acknowledgements
These SOPs were originally submitted by Sanford Stass, M.D. of the University of Maryland School of Medicine and William Grizzle of the University of Alabama at Birmingham School of Medicine. Modifications may have been made upon extensive discussions among the members of the EDRN Reference Laboratories and other scientific staff derived from the various groups within EDRN.
Announcement 10/23/2017

Three new FOAs on the Human Tumor Atlas and associated with the Cancer Moonshot Initiative have been released. Please click here for more information.

32nd SC Meeting
Thank you to everyone who made the 32nd EDRN Steering Committee meeting a success. The next event is the 10th EDRN Scientific Workshop from March 6-8, 2018 in Bethesda, MD. More information about this Workshop will be sent soon. Click here to view the flyer.
EDRN Founder Honored

Dr. Sudhir Srivastava was honored with the Distinguished Service Award from the American Pancreatic Association at the group's annual meeting this year, for his outstanding commitment to pancreatology.