Early Detection Research Network

Recommendations for the Generation, Quantification, Storage, and Handling of Peptides Used for Mass Spectrometry-Based Assays.

For many years, basic and clinical researchers have taken advantage of the analytical sensitivity and specificity afforded by mass spectrometry in the measurement of proteins. Clinical laboratories are now beginning to deploy these work flows as well. For assays that use proteolysis to generate peptides for protein quantification and characterization, synthetic stable isotope-labeled internal standard peptides are of central importance. No general recommendations are currently available surrounding the use of peptides in protein mass spectrometric assays.

The Clinical Proteomic Tumor Analysis Consortium of the National Cancer Institute has collaborated with clinical laboratorians, peptide manufacturers, metrologists, representatives of the pharmaceutical industry, and other professionals to develop a consensus set of recommendations for peptide procurement, characterization, storage, and handling, as well as approaches to the interpretation of the data generated by mass spectrometric protein assays. Additionally, the importance of carefully characterized reference materials-in particular, peptide standards for the improved concordance of amino acid analysis methods across the industry-is highlighted. The alignment of practices around the use of peptides and the transparency of sample preparation protocols should allow for the harmonization of peptide and protein quantification in research and clinical care.

Ackermann BL, Aebersold R, Barnidge DR, Boja E, Bunk DM, Carr SA, Chan DW, Chen J, Chen X, Clarke N, Crimmins DL, Davies SR, Ellis MJ, Fishman JB, Gao Y, Grant RP, Hiltke TR, Hoofnagle AN, Ketchum KA, Kinsinger CR, Kuhn E, Kusebauch U, Kushnir MM, Liebler DC, Liu T, Lowenthal MS, Massoni SA, Mesri M, Meyer MR, Moritz RL, Neubert H, Patterson SD, Paulovich AG, Qian WJ, Rockwood AL, Rodland KD, Rodriguez H, Rogers J, Schoenherr RM, Scott MG, Shi T, Singh RJ, Smith RD, Thomas SN, Townsend RR, Van Eyk JE, Whiteaker JR, Whiteley GR, Wong SH, Wrobel JA, Wu C, Zhang H, Zhang S, Zhang Z, Zimmerman LJ


Clin. Chem., 2016, 62 (1)

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