You are here: Home / Protocols / BRSCW Reference Set Application: Karen Abbott -University of Arkansas (2014)
Team Project

BRSCW Reference Set Application: Karen Abbott -University of Arkansas (2014)

395
Feng, ZidingFred Hutchinson Cancer Research Center
Assay-MagPix instrument using the magnetic spectral beads.
Glycomics
Breast and Gynecologic Cancers Research

Our earlier glycoproteomic studies have identified bisecting glycoslyation and core fucosylation changes on particular glycoproteins in endometrioid ovarian cancer tissues and plasma (Abbott et al, 2010, Proteomics). We have validated that these glycan changes occur on the same glycoproteins in serous ovarian cancer plasma using a lectin-pull down western blot assays. We would like to used pooled reference samples to develop a sensitive magnetic bead-based assay to detect these glycoproteins with bisecting and core fucosylation changes.

We would like to used pooled reference samples to develop a sensitive magnetic bead-based assay to detect these glycoproteins with bisecting and core fucosylation changes. We have purified fusion-tagged glycoproteins with the specific glycan structures that we will spike into the control samples to determine the range and variation of detection prior to the phase II sample analysis. We will also measure the variance between the malignant ovarian cancer pooled samples and control benign pooled samples to determine if the assay can measure statistically significant differences.
Data will be analyzed by the DMCC

There are currently no biomarkers annotated for this protocol.

No datasets are currently associated with this protocol.


Announcement 07/08/2019

Please visit the EDRN Registration page for registration/hotel information for the 11th EDRN Scientific Workshop in Bethesda, Maryland from September 10-12, 2019.
Announcement



 
This icon signifies that something is happening and we kindly ask you to please wait