QUANTITY: An Isobaric Tag for Quantitative Glycomics.


Glycan is an important class of macromolecules that play numerous biological functions. Quantitative glycomics--analysis of glycans at global level--however, is far behind genomics and proteomics owing to technical challenges associated with their chemical properties and structural complexity. As a result, technologies that can facilitate global glycan analysis are highly sought after. Here, we present QUANTITY (Quaternary Amine Containing Isobaric Tag for Glycan), a quantitative approach that can not only enhance detection of glycans by mass spectrometry, but also allow high-throughput glycomic analysis from multiple biological samples. This robust tool enabled us to accomplish glycomic survey of bioengineered Chinese Hamster Ovary (CHO) cells with knock-in/out enzymes involved in protein glycosylation. Our results demonstrated QUANTITY is an invaluable technique for glycan analysis and bioengineering.

  • Betenbaugh MJ
  • Chen L
  • Li S
  • Phinney KW
  • Song G
  • Turko IV
  • Wang M
  • Yang S
  • Yin B
  • Zhang H
PubMed ID
Appears In
Sci Rep, 2015, 5