The long non-coding RNA PCAT-1 promotes prostate cancer cell proliferation through cMyc.


Long non-coding RNAs (lncRNAs) represent an emerging layer of cancer biology, contributing to tumor proliferation, invasion, and metastasis. Here, we describe a role for the oncogenic lncRNA PCAT-1 in prostate cancer proliferation through cMyc. We find that PCAT-1-mediated proliferation is dependent on cMyc protein stabilization, and using expression profiling, we observed that cMyc is required for a subset of PCAT-1-induced expression changes. The PCAT-1-cMyc relationship is mediated through the post-transcriptional activity of the MYC 3' untranslated region, and we characterize a role for PCAT-1 in the disruption of MYC-targeting microRNAs. To further elucidate a role for post-transcriptional regulation, we demonstrate that targeting PCAT-1 with miR-3667-3p, which does not target MYC, is able to reverse the stabilization of cMyc by PCAT-1. This work establishes a basis for the oncogenic role of PCAT-1 in cancer cell proliferation and is the first study to implicate lncRNAs in the regulation of cMyc in prostate cancer.


One biomarker makes reference to this publication:

  • Cao Q
  • Chen W
  • Chinnaiyan AM
  • Evans JR
  • Feng FY
  • Han S
  • Iyer MK
  • Knudsen KE
  • Kothari V
  • Lawrence TS
  • Ljungman M
  • Paulsen MT
  • Prensner JR
PubMed ID
Appears In
Neoplasia, 2014, 16 (11)