Direct production and purification of T7 phage display cloned proteins selected and analyzed on microarrays.

Phage display technology has emerged into a powerful tool for identifying proteins with specific binding properties. This technology adds amino acid sequences to the carboxy terminus of a phage capsid protein, thus generating a fusion protein displayed on the surface of the phage. Here, we have developed a high-throughput strategy to synthesize purified protein that solves many of the problems associated with crude phage lysates. Phage DNA was used as a template for a nested PCR that added the T7 promoter, ribosome binding site, and a His6-tag. The PCR product was then used as a template for in vitro transcription/translation. The resulting His6-tagged recombinant protein was then purified by nickel affinity chromatography. The functionality of the purified protein was verified using protein microarray analysis.

Chatterjee M, Dryden SC, Mohapatra S, Nowak JE, Tainsky MA

16526412

BioTechniques, 2006, 40 (2)

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