Abbreviated Name

Liver Rapid Ref Set App (#2): Lubman (2010)

Lead Investigator

Lubman, David — University of Michigan Medical School

Coordinating Investigator

Feng, Ziding — Fred Hutchinson Cancer Center

Involved Investigators

• Feng, Ziding — Fred Hutchinson Cancer Center
• Lubman, David — University of Michigan Medical School

Abstact

Aims

The reference set will allow us to determine the best performance of the 5 glycoproteins by themselves or whether their combination has a better sensitivity and/or specificity and AUROC. While a direct comparison with AFP will be made, the reference set will not allow a robust comparison due to the low sample size.

Analytic Method

In this study, we used the χ2 and t test to compare the distribution differences of demographic and clinical variables among the two groups of cases and controls for discrete and continuous values, respectively. Spearman correlation test was applied to determine correlations of the tested values from different markers as well as the processing platforms. To justify performances for HCC early detection, the curves of receiver operating characteristic (ROC) were first plotted for all these markers. The area under the ROC curve (AUC) and the corresponding 95% confidence intervals (CI) were used to judge their overall performances. In addition, the logistic regression models with the forward selection were used to know if and how these biomarkers could be combined together to predictive positive responses. For the purpose to estimate their best performances, the potential cutoff values were proposed as all the empirical factor levels and their combination linear scores. The maximum sum value of sensitivity and specificity crossing different cutoff values was then employed to set up the optimal cutoff value. With the optimal cutoff value, the sensitivity, specificity and their 95% CIs were further calculated to contrast the resulting performances for these tested biomarkers

Biomarkers

• AFP

Data Collections

• No data available at this time for this protocol

Protocol ID

322

Protocol Type

Reference Set

Field of Research

Proteomics

Collaborative Group

G.I. and Other Associated Cancers Research Group

Cancer Types

• Liver cell carcinoma