Meltzer 3-marker panel for Esophageal Adenocarcinoma
|QA State:||Under Review|
Using real-time quantitative methylation-specific PCR, ten genes (HPP1, RUNX3, RIZ1, CRBP1, 3-OST-2, APC, TIMP3, p16, MGMT and p14) were screened for promoter hypermethylation in 77 esophageal adenocarcinoma, 93 Barrett's Esophagus, and 64 normal esophagus specimens. p16, RUNX3 and HPP1 displayed increasing methylation frequencies in Barrett's Esophagus vs. esophageal adenocarcinoma samples, with the increases in methylation occurring early, at the interface between Barrett's Esophagus and low-grade dysplasia. Further study has shown that hypermethylation of p16 and HPP1 are independently associated with an increased risk of progression. In combined analyses, risk was detectable up to, but not earlier than, two years prededing neoplastic progression. Hypermethylation of p16, RUNX3 and HPP1 in Barrett's Esophagus or low-grade dysplasia may represent independent risk factors for the progression of Barrett's Esophagus to high-grade dysplasia or esophageal adenocarcinoma.