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Immunoperoxidase Protocol for Dako Autostainer

  1. Cut tissue sections at 3-5 microns and mount on appropriate control box slides.
  2. Bake slides for 30 minutes in 70°C - 90°C oven.
  3. Label slides with necessary information including antibody.
  4. Deparaffinize slides in 3 changes of xylene 5 minutes each.
  5. Bring slides through 2 changes of absolute alcohol and 1 change of 95% alcohol 2 minutes each.
  6. Rinse in 2 changes of distilled waters. Then place in buffer solution.
  7. Perform any digestion or antigen retrieval protocols now if necessary.
  8. Set up DAKO autostainer with all reagents and antibodies necessary.
  9. Load slides and start machine.
  10. When completed, remove slides and wash in running tap water 2-5 minutes.
  11. Dehydrate slides

    Rinse 15-20 dips in

    • 2 changes 95% ETOH
    • 2 changes ABS ETOH
    • 3 changes Xylene
  12. Coverslip on automatic coverslipper.
Announcement 06/29/2009 

The RFA for the Biomarker Developmental Laboratories (RFA-CA-09-017: The Early Detection Research Network: Biomarker Developmental Laboratories (U01)) has been released. The Letter of Intent is due by September 29, 2009 and the application itself is due by October 29, 2009. Click the link for more information.

The 6th EDRN Scientific Workshop/19th EDRN Steering Committee Meeting in Bethesda, MD, takes place August 31-September 3, 2009. Please visit the Meeting Information and Registration page for details.

(Posted 2009-06-28)

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