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You are here: Home / Resources / EDRN Standard Operating Procedures (SOP) / Assays / Immunohistochemistry / Immunoperoxidase Protocol for Dako Autostainer

Immunoperoxidase Protocol for Dako Autostainer

  1. Cut tissue sections at 3-5 microns and mount on appropriate control box slides.
  2. Bake slides for 30 minutes in 70°C - 90°C oven.
  3. Label slides with necessary information including antibody.
  4. Deparaffinize slides in 3 changes of xylene 5 minutes each.
  5. Bring slides through 2 changes of absolute alcohol and 1 change of 95% alcohol 2 minutes each.
  6. Rinse in 2 changes of distilled waters. Then place in buffer solution.
  7. Perform any digestion or antigen retrieval protocols now if necessary.
  8. Set up DAKO autostainer with all reagents and antibodies necessary.
  9. Load slides and start machine.
  10. When completed, remove slides and wash in running tap water 2-5 minutes.
  11. Dehydrate slides

    Rinse 15-20 dips in

    • 2 changes 95% ETOH
    • 2 changes ABS ETOH
    • 3 changes Xylene
  12. Coverslip on automatic coverslipper.
2015 EDRN PI Orientation

The New and Continuing EDRN Principal Investigator Orientation will take place October 15–16, 2015 in Bethesda, Maryland.

Announcement 06/30/2015

A funding opportunity for a new pancreatic cancer initiative, called The Pancreatic Cancer Detection Consortium (U01), has been released. For more information, please go to http://grants.nih.gov /grants/guide/ pa-files/ PAR-15-289.html.

Announcement 04/08/2015

Thank you to everyone who made the 29th EDRN Steering Committee Meeting a success. The orientation for new and continuing EDRN PIs will be held October 15-16, 2015 on the NCI campus. More information will be available later this summer.