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You are here: Home / Resources / EDRN Standard Operating Procedures (SOP) / Assays / Immunohistochemistry / Immunoperoxidase Automated Procedure (Ventana)

Immunoperoxidase Automated Procedure (Ventana)

  1. Cut tissue sections at 3-5 microns and mount on appropriate control box slides.
  2. Bake slides for 30 minutes in 70°C - 90°C oven.
  3. Label slides with barcode labels indicating antibody.
  4. Place slides inside Ventana stainer and load all necessary antibodies and reagents.
  5. Start machine.
  6. Remove slides when finished and wash in warm soapy water (Dawn concentrate dishwashing detergent) to remove oil coating.
  7. Counter stain with Gills I hematoxylin for 1 minute then blue in Lithium Carbonate solution.
  8. Wash in tap water 2-5 minutes.
  9. Dehydrate slides.

    Rinse 15-20 dips in

    • 2 changes 95% ETOH
    • 2 changes ABS ETOH
    • 3 changes Xylene
  10. Coverslip on automatic coverslipper.
News

The final report of the 2013 Cancer Biomarkers Bioinformatics Workshop is now available.

Announcement 06/26/2014


Please click here to register for the 9th EDRN Scientific Workshop from September 8-10, 2014, in Bethesda, Maryland. The meeting registration page also has agendas and hotel reservation information.
Announcement 06/05/2014


Funding Opportunity Available

Both RFAs for Molecular and Cellular Characterization of Screen-Detected Lesions have been published.

RFA-CA-14-010.html

and

RFA-CA-14-011.html