Early Detection Research Network

Biomarkers: the key to early detection

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Immunofluorescence Staining: Direct immunofluorescence method is used to detect the deposit of immunoglobulins, complement components, fibrinogen, etc. in tissues. This technique is usually performed on frozen sections. The primary antibody is conjugated to fluorescein binds directly with the antigen and can be detected by the fluorescent tag using a fluorescent microscope.
Immunoperoxidase Staining: Pre-staining informational manual and the protocol documents.
Immunoperoxidase Automated Procedure (Ventana)
Immunoperoxidase Protocol for Dako Autostainer
Acknowledgements: These SOPs were originally submitted by Sanford Stass, M.D. of the University of Maryland School of Medicine and William Grizzle of the University of Alabama at Birmingham School of Medicine. Modifications may have been made upon extensive discussions among the members of the EDRN Reference Laboratories and other scientific staff derived from the various groups within EDRN.

: Announcement 04/16/2012

An EDRN Patient Advocacy Webinar is scheduled for Thursday, April 26th from 1-2 p.m. EDT. Space is limited; reserve your webinar seat at
GoToMeeting. (Please note that the link goes to a secure webpage.); Announcement

NASA has selected the EDRN Informatics Team to receive a Group Achievement Award. The award will be given to the teams at NCI, DMCC, Dartmouth and JPL. These are NASA-wide awards and selected by an awards committee appointed by NASA Headquarters. The citation is as follows: For the innovative and pioneering use of NASA data system technologies to construct a national bioinformatics network of cancer biomarker research.