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Immunohistochemistry

Immunofluorescence Staining
Direct immunofluorescence method is used to detect the deposit of immunoglobulins, complement components, fibrinogen, etc. in tissues. This technique is usually performed on frozen sections. The primary antibody is conjugated to fluorescein binds directly with the antigen and can be detected by the fluorescent tag using a fluorescent microscope.
Immunoperoxidase Staining
Pre-staining informational manual and the protocol documents.
Immunoperoxidase Automated Procedure (Ventana)
Immunoperoxidase Protocol for Dako Autostainer
Acknowledgements
These SOPs were originally submitted by Sanford Stass, M.D. of the University of Maryland School of Medicine and William Grizzle of the University of Alabama at Birmingham School of Medicine. Modifications may have been made upon extensive discussions among the members of the EDRN Reference Laboratories and other scientific staff derived from the various groups within EDRN.
2015 EDRN PI Orientation

The New and Continuing EDRN Principal Investigator Orientation will take place October 15–16, 2015 in Bethesda, Maryland.

Announcement 06/30/2015

A funding opportunity for a new pancreatic cancer initiative, called The Pancreatic Cancer Detection Consortium (U01), has been released. For more information, please go to http://grants.nih.gov /grants/guide/ pa-files/ PAR-15-289.html.

Announcement 04/08/2015

Thank you to everyone who made the 29th EDRN Steering Committee Meeting a success. The orientation for new and continuing EDRN PIs will be held October 15-16, 2015 on the NCI campus. More information will be available later this summer.