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Hybridomas for Pancreatic Cancer Biomarkers

Background

The National Cancer Institute Early Detection Research Network (EDRN) is collaborating with the Lustgarten Foundation for Pancreatic Cancer Research (Lustgarten Foundation), a non-profit corporation dedicated to advancing research related to the diagnosis, treatment, cure, and prevention of pancreatic cancer. The Lustgarten Foundation has initiated The Pancreatic Cancer Biomarker Development Program to create mouse monoclonal antibodies against promising biomarker targets for pancreatic cancer. Under this program, the Lustgarten Foundation will fund four leading cancer research organizations including the Dana-Farber Cancer Institute, the University of California at San Francisco, the Van Andel Research Institute, and the Canary Foundation (collectively referred to as the Consortium). The Consortium members will generate at least two mouse monoclonal antibodies to different epitopes on each target. Each monoclonal antibody is produced by a hybridoma cell line which allows for a renewable source of the antibody. The program initiated by the Lustgarten Foundation will result in a collection of monoclonal antibodies to pancreatic cancer biomarkers that will be useful for the development of novel diagnostic tests for this disease. In particular, the antibodies may be part of an enzyme-linked immunosorbent assay (ELISA) for the detection of pancreatic cancer. The antibodies may be used for detecting proteins released in sera or they may be used for immunohistochemistry of tumor samples.

The Lustgarten Foundation is making a significant investment to generate this large collection of monoclonal antibodies against pancreatic cancer biomarker targets. The value of this collection of antibodies to public health cannot be fully realized without 1) a common storage site for the hybridoma cell lines and 2) a uniform system for depositing and accessing the hybridomas. The NCI is uniquely qualified to provide 1) the capacity for storage of the collection of hybridoma cell lines and 2) the experience and expertise needed for effective distribution of the hybridomas. The NCI EDRN assumes the responsibility for sharing and distributing the antibody resources to extramural investigators who meet the goals of EDRN.

Requesting Hybridomas

To request any of these hybridomas submit a concise description of which hybridomas you wish to receive, the purpose for using the antibodies, and what you wish to accomplish with them. The EDRN has a review panel established to assess the research needs for these requests and should provide a prompt review. Please be aware that after obtaining approval to receive hybridomas a Material Transfer Agreement (MTA) with the NCI will need to be established before they can be sent out. Submit all requests to: kruegerk@mail.nih.gov

GeneProtein Name# Hybridomas Available
AGR2 Anterior Grade Protein 2 2
CEACAM7 Carcinoembryonic Antigen Cell Adhesion Molecule 7 2
CST4 Cystatin-S 2
LGALS7 Galectin-7 2
GP2 Glycoprotein 2 2
HPCAL1 Hippocalcin-like protein 1 2
MATN3 Matrilin-3 1
MMP14 Matrix Metalloproteinase 14 1
PEBP1 Phosphatidylethanolamine Binding Protein 4
PSG11 Pregnancy Specific beta-1 Glycoprotein 11 2
PSCA Prostate Stem Cell Antigen 2
PP1C Protein Phosphatase 1 2
REG3A Regenerating Islet-derived Protein 3 alpha 2
REG3G Regenerating Islet-derived Protein 3 gamma 2
SERPINE2 Serpin Peptidase Inhibitor 2
SPARCL1 SPARC-like Protein 1 2
SRPX2 Sushi Repeat-containing Protein 1
TACSTD2 Tumor-associated Calcium Signal Transducer 2 2
WISP3 WNT1-inducible Signaling Pathway Protein 3 2
C9orf19 (open reading frame) 1
2015 Steering Committee Meeting

The next EDRN Steering Committee Meeting will take place March 31st through April 2nd, 2015, in Atlanta, Georgia.

Announcement 12/02/2014

New Round of EDRN FOAs

The RFAs for EDRN have been released:
- Biomarker Developmental Laboratories (U01),
- Clinical Validation Centers (U01),
- Biomarker Reference Laboratories (U24),
- Data Management and Coordinating Center (U24).

EDRN Renewal flyer NOTE-New receipt deadline for applications submitted for all EDRN FOAs is January 20, 2015, by 5:00 PM local time of applicant organization.

There will be a Pre-Application webinar to discuss each of the four individual EDRN FOAs on Tuesday, December 2nd, 2014, from 1pm-5pm (Eastern). Potential applicants interested in participating in the webinar should send a message to Dr. Sharmistha Ghosh (ghoshjanjigias@mail.nih.gov) no later than 5:00 p.m. (EST) November 21, 2014. Please mention the FOA of interest in the subject line.

Announcement 10/07/2014

EDRN Patient Advocates will host an EDRN Advocacy Educational Webinar, Biomarkers for Prostate Cancer Detection and Monitoring, on Monday, January 12th, 2015, at 1 p.m. EDT / 10 a.m. PDT. Registration is not required for this. Please click for more information.