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Biomarker Detection Using NAPPA Tumor Antigen Arrays: EDRN Supplement

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Anderson, KarenArizona State University

No coordinating investigator defined.

Broad based screen of possible biomarkers. p53 is included here.
Case/control
Proteomics
Breast and Gynecologic Cancers Research

The overall goal of this project application for the EDRN set-aside funds is to focus our collaborative efforts to identify p53 mutation-specific antibody biomarkers in breast, prostate, and ovarian cancer. P53-specific gene mutations are frequent in multiple cancer types. Of the common solid tumors, p53 mutations have been identified in 50% of lung and ovarian cancers, 45% of colon cancers, 20% of breast cancers, and 10-30% of prostate cancers (The p53 Mutation Handbook, T. Soussi, http://p53/free/fr). The most common mutations vary from cancer to cancer, with 50 point mutations covering the 10 most common mutations for all major solid tumors

1.B. Project Objectives Aim 1: Generation of NAPPA protein microarrays expressing the fifty most common p53 point mutations for solid tumors as well as deletion constructs of p53. Aim 2: Identify p53 mutation-specific antibodies in a test set of breast, prostate, and ovarian cancer patient sera. a.   Using test sera from patients with prostate, ovarian, and breast cancers, identify patient sera that contain anti-p53 antibodies. b.   Using p53-antibody-positive sera from patients, screen the p53 mutation microarray to identify mutation-specific and domain-specific antibodies c.   Using p53-antibody-negative sera from patients, screen the p53 mutation microarray to identify mutation-specific antibodies d.   Determine the sensitivity and specificity of individual antibodies that distinguish patient sera from normal sera. e.   Determine if mutation-specific antibodies can distinguish tumor types.
Various including quantile regression, Fishers exact, binomial proportion and others. The methods are not yet fixed because we are still in discovery.

There are currently no biomarkers annotated for this protocol.

No datasets are currently associated with this protocol.


Announcement 09/14/2014

Thank you to everyone who helped make the 9th EDRN Scientific Workshop a success. We look forward to seeing everyone at the 28th EDRN Steering Committee Meeting from March 31-April 2, 2015, in Atlanta, GA.

Announcement 06/05/2014


Funding Opportunity Available

Both RFAs for Molecular and Cellular Characterization of Screen-Detected Lesions have been published.

RFA-CA-14-010.html

and

RFA-CA-14-011.html